Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli
Stenotrophomonas maltophilia
KM Value [mM]
0.00514
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.00712
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.008
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0088
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Organism
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
ATP + (2R,3R)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4R)-3-hexyl-4-heptyloxetan-2-one
?
ATP + (2R,3S)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4S)-3-hexyl-4-heptyloxetan-2-one
?
ATP + (2S,3R)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4R)-3-hexyl-4-heptyloxetan-2-one
?
ATP + (2S,3S)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4S)-3-hexyl-4-heptyloxetan-2-one
?
Synonyms
oleC
Stenotrophomonas maltophilia
Turnover Number [1/s]
0.006
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.01
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0245
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0301
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
expression in Escherichia coli
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
0.00514
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.00712
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.008
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0088
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
ATP + (2R,3R)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4R)-3-hexyl-4-heptyloxetan-2-one
?
ATP + (2R,3S)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4S)-3-hexyl-4-heptyloxetan-2-one
?
ATP + (2S,3R)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4R)-3-hexyl-4-heptyloxetan-2-one
?
ATP + (2S,3S)-2-hexyl-3-hydroxydecanoic acid
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4S)-3-hexyl-4-heptyloxetan-2-one
?
Turnover Number [1/s] (protein specific)
0.006
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.01
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0245
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0301
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
General Information
physiological function
the final step in the bacterial pathway of olefin biosynthesis is an OleC-catalyzed ATP-dependent decarboxylative dehydration to form the corresponding Z olefins. Higher kcat/Km values are seen for substrates with longer alkyl chains. All four stereoisomers of 2-hexyl-3-hydroxydecanoic acid are substrates, and the final product, after decarboxylation, in each case is (Z)-pentadec-7-ene
Stenotrophomonas maltophilia
General Information (protein specific)
physiological function
the final step in the bacterial pathway of olefin biosynthesis is an OleC-catalyzed ATP-dependent decarboxylative dehydration to form the corresponding Z olefins. Higher kcat/Km values are seen for substrates with longer alkyl chains. All four stereoisomers of 2-hexyl-3-hydroxydecanoic acid are substrates, and the final product, after decarboxylation, in each case is (Z)-pentadec-7-ene
Stenotrophomonas maltophilia
KCat/KM [mM/s]
1.33
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
1.5
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.12
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.5
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
KCat/KM [mM/s] (protein specific)
1.33
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
1.5
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.12
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.5
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Other publictions for EC 6.1.3.1
741939
Christenson
beta-Lactone synthetase found ...
Arenimonas malthae, Lysobacter dokdonensis, Micrococcus luteus, Micrococcus luteus ATCC4698, Stenotrophomonas maltophilia, Xanthomonas campestris
Biochemistry
56
348-351
2017
-
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-
-
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-
-
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12
-
6
-
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-
-
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18
-
5
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12
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18
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5
5
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742797
Christenson
Active multienzyme assemblies ...
Xanthomonas campestris
J. Bacteriol.
199
e00890
2017
-
-
1
-
-
-
-
-
-
-
1
-
-
1
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1
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1
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1
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1
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1
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1
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742283
Kancharla
Stenotrophomonas maltophilia ...
Stenotrophomonas maltophilia
ChemBioChem
17
1426-1429
2016
-
-
1
-
-
-
-
4
-
-
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-
1
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4
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1
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4
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1
-
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4
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-
4
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-
4
-
-
-
-
-
1
1
-
4
4
741673
Sukovich
Structure, function, and insi ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Appl. Environ. Microbiol.
76
3842-3849
2010
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2
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1
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