Stenotrophomonas maltophilia OleC-catalyzed ATP-dependent formation of long-chain Z-olefins from 2-alkyl-3-hydroxyalkanoic acids
Kancharla, P.; Bonnett, S.A.; Reynolds, K.A.; ChemBioChem 17, 1426-1429 (2016) 
Data extracted from this reference:
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00514
-
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.00712
-
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.008
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0088
-
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2R,3R)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4R)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
ATP + (2R,3S)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4S)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
ATP + (2S,3R)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4R)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
ATP + (2S,3S)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4S)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.01
-
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0245
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0301
-
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00514
-
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.00712
-
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.008
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0088
-
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2R,3R)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4R)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
ATP + (2R,3S)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3R,4S)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
ATP + (2S,3R)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4R)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
ATP + (2S,3S)-2-hexyl-3-hydroxydecanoic acid
-
742283
Stenotrophomonas maltophilia
AMP + diphosphate + (3S,4S)-3-hexyl-4-heptyloxetan-2-one
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.006
-
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.01
-
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0245
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
0.0301
-
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
General Information
General Information
Commentary
Organism
physiological function
the final step in the bacterial pathway of olefin biosynthesis is an OleC-catalyzed ATP-dependent decarboxylative dehydration to form the corresponding Z olefins. Higher kcat/Km values are seen for substrates with longer alkyl chains. All four stereoisomers of 2-hexyl-3-hydroxydecanoic acid are substrates, and the final product, after decarboxylation, in each case is (Z)-pentadec-7-ene
Stenotrophomonas maltophilia
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the final step in the bacterial pathway of olefin biosynthesis is an OleC-catalyzed ATP-dependent decarboxylative dehydration to form the corresponding Z olefins. Higher kcat/Km values are seen for substrates with longer alkyl chains. All four stereoisomers of 2-hexyl-3-hydroxydecanoic acid are substrates, and the final product, after decarboxylation, in each case is (Z)-pentadec-7-ene
Stenotrophomonas maltophilia
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.33
-
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
1.5
-
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.12
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.5
-
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.33
-
(2S,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
1.5
-
(2R,3R)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.12
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
3.5
-
(2S,3S)-2-hexyl-3-hydroxydecanoic acid
pH 7.5, 25°C
Stenotrophomonas maltophilia
Other publictions for EC 6.1.3.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741939
Christenson
beta-Lactone synthetase found ...
Arenimonas malthae, Lysobacter dokdonensis, Micrococcus luteus, Micrococcus luteus ATCC4698, Stenotrophomonas maltophilia, Xanthomonas campestris
Biochemistry
56
348-351
2017
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12
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6
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18
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12
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18
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5
5
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742797
Christenson
Active multienzyme assemblies ...
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199
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1
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1
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1
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1
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1
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1
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1
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1
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1
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742283
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Stenotrophomonas maltophilia ...
Stenotrophomonas maltophilia
ChemBioChem
17
1426-1429
2016
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1
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4
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1
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4
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4
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1
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4
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4
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4
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1
1
-
4
4
741673
Sukovich
Structure, function, and insi ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Appl. Environ. Microbiol.
76
3842-3849
2010
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2
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