Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | elongation factor Tu has no stimulating effect on the enzyme with both substrates: tRNAAla and Ala-tmRNA, but it protects both acylated RNAs against hydrolysis of the alanyl ester bond | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, ternary complex formation | Escherichia coli | |
0.0015 | - |
tRNAAla | pH 7.6, 37°C, recombinant His-tagged enzyme | Escherichia coli | |
0.024 | - |
tmRNA | pH 7.6, 37°C, recombinant His-tagged enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
1 * 96000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAAla | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
purified recombinant His-tagged enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tmRNA | transfer messenger RNA, 75fold reduced activity compared to cognate tRNAAla | Escherichia coli | AMP + diphosphate + L-alanyl-tmRNA | - |
? | |
ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? | |
ATP + L-alanine + tRNAAla | 2-step reaction | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 96000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Ala-tRNA synthetase | - |
Escherichia coli |
Alanine transfer RNA synthetase | - |
Escherichia coli |
Alanine translase | - |
Escherichia coli |
Alanine tRNA synthetase | - |
Escherichia coli |
Alanine-transfer RNA ligase | - |
Escherichia coli |
alanine-tRNA ligase | - |
Escherichia coli |
Alanyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Alanyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Alanyl-transfer RNA synthetase | - |
Escherichia coli |
Alanyl-tRNA synthetase | - |
Escherichia coli |
AlaRS | - |
Escherichia coli |
Synthase, alanyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
tmRNA | pH 7.6, 37°C, 0.001 mg recombinant His-tagged enzyme | Escherichia coli | |
0.2 | - |
tRNAAla | pH 7.6, 37°C, 0.00025 mg recombinant His-tagged enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |