KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00061 | - |
tRNALeu | 37°C, pH 7.8 | Aquifex aeolicus | |
0.0012 | - |
tRNALeuA35G | 37°C, pH 7.8 | Aquifex aeolicus | |
0.011 | - |
tRNALeuA73G | 37°C, pH 7.8 | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeuA35G | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeuA35G | - |
? | |
ATP + L-leucine + tRNALeuA73G | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeuA73G | - |
? | |
additional information | aminoacylation of minihelices is strongly dependent on the presence of the A73 identity nucleotide and greatly stimulated by destabilization of the first base pair. Addition of RNA helices that mimic the anticodon domain stimulates minihelixLeu charging by alphabeta-LeuRS indicating possible domain-domain communication. MinihelixLeu cannot be misaminoacylated, perhaps because of the tRNA-independent pretransfer editing activity of alphabeta-LeuRS | Aquifex aeolicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
tRNALeuA73G | 37°C, pH 7.8 | Aquifex aeolicus | |
0.31 | - |
tRNALeuA35G | 37°C, pH 7.8 | Aquifex aeolicus | |
0.5 | - |
tRNALeu | 37°C, pH 7.8 | Aquifex aeolicus |