Cloned (Comment) | Organism |
---|---|
gene pylS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Desulfitobacterium hafniense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of pyrrolysylation of several mutant tRNAPyls, overview | Desulfitobacterium hafniense | |
0.0029 | - |
tRNAPyl | pH 7.2, 37°C, wild-type tRNAPyl | Desulfitobacterium hafniense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Desulfitobacterium hafniense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-pyrrolysine + tRNAPyl | Desulfitobacterium hafniense | pyrrolysyl-tRNA synthetase PylRS attaches L-pyrrolysine to its cognate tRNA, the special amber suppressor tRNAPyl, encoded by gene pylT | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfitobacterium hafniense | - |
gene pylS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Desulfitobacterium hafniense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-pyrrolysine + tRNAPyl | pyrrolysyl-tRNA synthetase PylRS attaches L-pyrrolysine to its cognate tRNA, the special amber suppressor tRNAPyl, encoded by gene pylT | Desulfitobacterium hafniense | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
ATP + L-pyrrolysine + tRNAPyl | PylRS binds tRNA predominantly along the phosphate backbone of the T-loop, the D-stem and the acceptor stem, while no significant contacts with the anticodon arm occur, the tRNAPyl anticodon is not important for recognition by bacterial PylRS, overview | Desulfitobacterium hafniense | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
ATP + N-epsilon-cyclopentyloxycarbonyl-L-lysine + tRNAPyl | substrate specificity, ability of 24 mutant tRNA species to be aminoacylated by the enzyme with the pyrrolysine analog N-epsilon-cyclopentyloxycarbonyl-L-lysine, overview, the discriminator base G73 and the first base pair G1-C72 in the acceptor stem are major identity elements | Desulfitobacterium hafniense | AMP + diphosphate + N-epsilon-cyclopentyloxycarbonyl-L-lysyl-tRNAPyl | - |
? | |
additional information | enzyme activity with wild-type and different mutant tRNAPyls, overview, 3'-labeled tRNAPyl footprinting with S1 and T1 nuclease digestions, overview | Desulfitobacterium hafniense | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PylRS | - |
Desulfitobacterium hafniense |
pyrrolysyl-tRNA synthetase | - |
Desulfitobacterium hafniense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Desulfitobacterium hafniense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
tRNAPyl | pH 7.2, 37°C, wild-type tRNAPyl | Desulfitobacterium hafniense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Desulfitobacterium hafniense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Desulfitobacterium hafniense |