Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
apo, L-arginine-complexed, and L-canavanine-complexed forms of the cytoplasmic free enzyme isoform, hanging drop vapor diffusion method, using 0.085 mM sodium citrate tribasic dehydrate (pH 5.7), 24% (w/v) polyethylene glycol 4000, 0.17 M ammonium acetate, and 15% (v/v) glycerol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Homo sapiens | - |
AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P54136 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arginyl-tRNA synthetase | - |
Homo sapiens |
ArgRS | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |