Cloned (Comment) | Organism |
---|---|
gene argS, DNA and amino acid sequence determination and analysis, expression of N-terminally HIS-/thioredoxin-tagged enzyme in Escherichia coli strain BL21 | Canavalia ensiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 50% inhibition at 100 mM, recombinant HIS-/thioredoxin-tagged enzyme | Canavalia ensiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
tRNAArg ACG | pH 7.5, 30°C, recombinant detagged enzyme | Canavalia ensiformis | |
0.00184 | - |
tRNAArg ACG | pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme | Canavalia ensiformis | |
0.0164 | - |
L-arginine | pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme | Canavalia ensiformis | |
0.0196 | - |
L-arginine | pH 7.5, 30°C, recombinant detagged enzyme | Canavalia ensiformis | |
0.186 | - |
ATP | pH 7.5, 30°C, recombinant detagged enzyme | Canavalia ensiformis | |
0.207 | - |
ATP | pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme | Canavalia ensiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Canavalia ensiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Canavalia ensiformis | - |
AMP + diphosphate + L-arginyl tRNAArg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canavalia ensiformis | B2G3G6 | gene argS | - |
Purification (Comment) | Organism |
---|---|
soluble recombinant N-terminally HIS-/thioredoxin-tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography, tag cleavage by thrombin | Canavalia ensiformis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | enzyme expression | Canavalia ensiformis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of recombinant tagged and detagged enzyme | Canavalia ensiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg ACG | tRNA substrate from Escherichia coli | Canavalia ensiformis | AMP + diphosphate + L-arginyl tRNAArg ACG | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arginyl-tRNA synthetase | - |
Canavalia ensiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Canavalia ensiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.215 | - |
L-arginine | pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme | Canavalia ensiformis | |
0.337 | - |
L-arginine | pH 7.5, 30°C, recombinant detagged enzyme | Canavalia ensiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
about | Canavalia ensiformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | activity range, pH-profile, overview | Canavalia ensiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Canavalia ensiformis |