KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
tRNAArg | ATP-diphosphate exchange, pH 6.0 | Geobacillus stearothermophilus | |
0.0002 | - |
tRNAArg | substrate from E. coli, , ATP-diphosphate exchange, pH 6.0 | Geobacillus stearothermophilus | |
0.00031 | - |
tRNAArg | aminoacylation | Geobacillus stearothermophilus | |
0.0019 | - |
Arg | aminoacylation | Geobacillus stearothermophilus | |
0.0025 | - |
tRNAArg | pH 7.4, ATP-diphosphate exchange | Geobacillus stearothermophilus | |
0.0036 | - |
tRNAArg | substrate from E. coli, , ATP-diphosphate exchange, pH 7.4 | Geobacillus stearothermophilus | |
0.54 | - |
ATP | aminoacylation | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus NCA 1518 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-25°C, less than 30% loss of activity after 6 months | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Bacillus stearothermophilus tRNAArg, E. coli tRNAArg and yeast tRNAArg (poor) | Geobacillus stearothermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | nucleotides other than ATP are not effective | Geobacillus stearothermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | Bacillus stearothermophilus tRNAArg, E. coli tRNAArg and yeast tRNAArg (poor) | Geobacillus stearothermophilus NCA 1518 | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | nucleotides other than ATP are not effective | Geobacillus stearothermophilus NCA 1518 | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
additional information | arginine-dependent ATP-diphosphate exchange | Geobacillus stearothermophilus | ? | - |
? | |
additional information | arginine-dependent ATP-diphosphate exchange | Geobacillus stearothermophilus NCA 1518 | ? | - |
? |