Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified His-tagged wild-type and S581L mutant enzymes, sitting drop vapour diffusion nanocrystallization method, mutant S581L GlyRS from 20% PEG 3350, 0.2 M Na2SO4, 0.1 M Bis-Tris propane, pH 6.5, wild-type GlyRS from 20% PEG 3350, 0.2M sodium bromide, 0.1 M bis-Tris propane pH 8.5, X-ray diffraction structure determination and anaylsis at 2.8A and 3.1 A resolution, respectively | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S581L | the naturally occuring dominant mutation to reduced aminoacylation activity and to motor nerve degeneration | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | Homo sapiens | enzyme mutations with reduced enzyme activity cause distal spinal muscular atrophy type V or Charcot-Marie-Tooth type 2D, i.e. dSMAV/CMT2D | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene GARS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | enzyme mutations with reduced enzyme activity cause distal spinal muscular atrophy type V or Charcot-Marie-Tooth type 2D, i.e. dSMAV/CMT2D | Homo sapiens | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
ATP + glycine + tRNAGly | tRNA binding site structure, and wild-type and S581L mutant active site structures, overview | Homo sapiens | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glycyl-tRNA synthetase | - |
Homo sapiens |
GlyRS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |