Literature summary for 5.6.2.4 extracted from

Suntornthiticharoen, P.; Petmitr, S.; Chavalitshewinkoon-Petmitr, P.
Purification and characterization of a novel 3-5 DNA helicase from Plasmodium falciparum and its sensitivity to anthracycline antibiotics (2006), Parasitology, 133, 389-398 .

Search for more literature for 5.6.2.4

Inhibitors

Inhibitors	Comment	Organism	Structure
Aclarubicin	-	Plasmodium falciparum
daunorubicin	-	Plasmodium falciparum
doxorubicin	-	Plasmodium falciparum
EDTA	the enzyme activity is inhibited by 5 mM EDTA	Plasmodium falciparum
KCl	inhibited by 200 mM KCl	Plasmodium falciparum
NaCl	inhibited by 200 mM NaCl	Plasmodium falciparum
Nogalamycin	-	Plasmodium falciparum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	unwinding activity requires Mg2+, 2 mM used in assay conditions	Plasmodium falciparum
additional information	not affected by Ca2+ or Zn2+	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Resource Q column chromatography, HiTrap heparin column chromatography Mono S column chromatography and single-stranded DNA column chromatography	Plasmodium falciparum

Storage Stability

Storage Stability	Organism
-70°C, purified enzyme, 6 months, 25% loss of activity	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the purified enzyme is unable to unwind blunt-ended duplex DNA substrate or 31-mer partial duplex substrate with hanging tail at 5'- or 3'-end	Plasmodium falciparum	?	-	-
partial duplex DNA	32P-labelled oligodeoxynucleotide (17 mer, 5'-GTAAAACGACGGCCAGT-3') annealed to single-stranded M13mp19 (+) DNA. The enzyme moves unidirectionally in the 3' to 5' direction along the bound strand and prefers a fork-like substrate structure	Plasmodium falciparum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 90000, SDS-PAGE	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
DHA	-	Plasmodium falciparum

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Plasmodium falciparum
dATP	dATP supports unwinding at 42% of the efficiency of ATP	Plasmodium falciparum

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.002	-	at pH 9.0 and 37°C	Plasmodium falciparum	daunorubicin
0.005	-	at pH 9.0 and 37°C	Plasmodium falciparum	Nogalamycin
0.008	-	at pH 9.0 and 37°C	Plasmodium falciparum	doxorubicin
0.009	-	at pH 9.0 and 37°C	Plasmodium falciparum	Aclarubicin

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)