Literature summary for 5.6.2.4 extracted from

Brewster, A.S.; Slaymaker, I.M.; Afif. S.A.; Chen, X.S.
Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding (2010), BMC Mol. Biol., 11, 62.

Search for more literature for 5.6.2.4

Protein Variants

Protein Variants	Comment	Organism
D327A	about 75% of wild-type helicase activity	Saccharolobus solfataricus
E326A	about 95% of wild-type helicase activity	Saccharolobus solfataricus
E326A/D327A	about 85% of wild-type helicase activity	Saccharolobus solfataricus
K323A	about 40% of wild-type helicase activity	Saccharolobus solfataricus
K346A	no ATPase activity, no helicase activity	Saccharolobus solfataricus
L325A	about 40% of wild-type helicase activity	Saccharolobus solfataricus
R329A	about 50% of wild-type helicase activity	Saccharolobus solfataricus
R331A	no ATPase activity, no helicase activity	Saccharolobus solfataricus
V324A	about 90% of wild-type helicase activity	Saccharolobus solfataricus
V324A/L325A	about 80% of wild-type helicase activity	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00006	-	ATP	pH and temperature not specified in the publication, mutant enzyme E326A	Saccharolobus solfataricus
0.00009	-	ATP	pH and temperature not specified in the publication, mutant enzyme E326A/D327A	Saccharolobus solfataricus
0.0001	-	ATP	pH and temperature not specified in the publication, mutant enzyme D327A	Saccharolobus solfataricus
0.00012	-	ATP	pH and temperature not specified in the publication, mutant enzyme V324A	Saccharolobus solfataricus
0.00014	-	ATP	pH and temperature not specified in the publication, mutant enzyme K323A	Saccharolobus solfataricus
0.00017	-	ATP	pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus
0.00017	-	ATP	pH and temperature not specified in the publication, mutant enzyme R329A	Saccharolobus solfataricus
0.00023	-	ATP	pH and temperature not specified in the publication, mutant enzyme V324A/L325A	Saccharolobus solfataricus
0.00027	-	ATP	pH and temperature not specified in the publication, mutant enzyme L325A	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q9UXG1	-	-
Saccharolobus solfataricus P2	Q9UXG1	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	the protein exterior hairpin reveals critical residues for helicase activity and DNA binding	Saccharolobus solfataricus	ADP + phosphate	-	?
ATP + H2O	the protein exterior hairpin reveals critical residues for helicase activity and DNA binding	Saccharolobus solfataricus P2	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	-	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
mini-chromosome maintenance protein	-	Saccharolobus solfataricus
SsoMCM	-	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	ATP	pH and temperature not specified in the publication, mutant enzyme K323A	Saccharolobus solfataricus
0.03	-	ATP	pH and temperature not specified in the publication, mutant enzyme R329A	Saccharolobus solfataricus
0.033	-	ATP	pH and temperature not specified in the publication, mutant enzyme E326A/D327A	Saccharolobus solfataricus
0.038	-	ATP	pH and temperature not specified in the publication, mutant enzyme D327A	Saccharolobus solfataricus
0.04	-	ATP	pH and temperature not specified in the publication, mutant enzyme V324A	Saccharolobus solfataricus
0.048	-	ATP	pH and temperature not specified in the publication, mutant enzyme E326A	Saccharolobus solfataricus
0.065	-	ATP	pH and temperature not specified in the publication, mutant enzyme V324A/L325A	Saccharolobus solfataricus
0.067	-	ATP	pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus
0.08	-	ATP	pH and temperature not specified in the publication, mutant enzyme L325A	Saccharolobus solfataricus

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Release 2023.1 (January 2023)