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Literature summary for 5.6.2.4 extracted from

  • Balasingham, S.; Zegeye, D.; Homberset, H.; Rossi, M.; Laerdahl, J.; Bohr, V.; Tonjum, T.
    Enzymatic activities and DNA substrate specificity of Mycobacterium tuberculosis DNA helicase XPB (2012), PLoS ONE, 7, e36960.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mycobacterium tuberculosis
Mn2+
-
 Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Mycobacterium tuberculosis ADP + phosphate
-
 ?
dATP + H2O
-
 Mycobacterium tuberculosis dADP + phosphate
-
 ?
additional information Mtb XPB efficiently catalyzes DNA unwinding. Enzyme requires a DNA substrate with a 39 overhang of 15 nucleotides or more. Enzyme is not active on substrates containing a 39 RNA tail. Mtb XPB efficiently catalyzes ATP-independent annealing of complementary DNA strands Mycobacterium tuberculosis ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA helicase XPB
-
 Mycobacterium tuberculosis
Mtb XPB
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mycobacterium tuberculosis