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Literature summary for 5.6.2.4 extracted from
- Purification and characterization of a novel 3'-5' DNA helicase from Plasmodium falciparum and its sensitivity to anthracycline antibiotics (2006), Parasitology, 133, 389-398.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aclarubicin | - |
Plasmodium falciparum |  |
| daunorubicin | - |
Plasmodium falciparum | |
| doxorubicin | - |
Plasmodium falciparum | |
| EDTA | 5 mM | Plasmodium falciparum | |
| KCl | 200 mM, inhibits | Plasmodium falciparum | |
| NaCl | 200 mM, inhibits | Plasmodium falciparum | |
| Nogalamycin | - |
Plasmodium falciparum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | unwinding activity requires Mg2+ | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90000, SDS-PAGE | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Plasmodium falciparum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, loses 25% of its activity following storage for 6 months | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | the enzyme moves unidirectionally in the 3' to 5' direction along the bound strand and prefers a fork-like substrate structure and could not unwind blunt-ended duplex DNA | Plasmodium falciparum | ADP + phosphate | - |
? | |
| dATP + H2O | the enzyme moves unidirectionally in the 3' to 5' direction along the bound strand and prefers a fork-like substrate structure and could not unwind blunt-ended duplex DNA. dATP supports unwinding at 42% of the efficiency of ATP | Plasmodium falciparum | dADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3'-5' DNA helicase | - |
Plasmodium falciparum |
| 3'-5' PfDH | - |
Plasmodium falciparum |
| PfDH A | - |
Plasmodium falciparum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
pH 9.0, 37°C | Plasmodium falciparum | daunorubicin | |
| 0.005 | - |
pH 9.0, 37°C | Plasmodium falciparum | Nogalamycin | |
| 0.009 | - |
pH 9.0, 37°C | Plasmodium falciparum | doxorubicin | |
| 0.009 | - |
pH 9.0, 37°C | Plasmodium falciparum | Aclarubicin | |
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