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Literature summary for 5.6.2.4 extracted from

  • Bennett, R.J.; Sharp, J.A.; Wang, J.C.
    Purification and characterization of the Sgs1 DNA helicase activity of Saccharomyces cerevisiae (1998), J. Biol. Chem., 273, 9644-9650.
    View publication on PubMed
Search for more literature for 5.6.2.4

Activating Compound

Activating Compound Comment Organism Structure
single-stranded DNA-binding protein of Escherichia coli, stimulates activity Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
a recombinant Sgs1 fragment (amino acids 400–1268 of the 1447-amino acid full-length protein) is overexpressed in yeast Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P35187
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Sgs1 fragment (amino acids 400–1268 of the 1447-amino acid full-length protein) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O exhibits an ATPase activity in the presence of single- or double-stranded DNA. Displacement of the DNA strand occurs in the 3' to 5' direction with respect to the single-stranded DNA flanking the duplex. The efficiency of unwinding is found to correlate inversely with the length of the duplex region. The recombinant Sgs1 fragment is found to bind more tightly to a forked DNA substrate than to either single or double-stranded DNA. Like the DNA-DNA helicase activity, unwinding of the DNA-RNA hybrid is driven by the hydrolysis of ATP or dATP Saccharomyces cerevisiae ADP + phosphate
-
 ?
dATP + H2O exhibits an ATPase activity in the presence of single- or double-stranded DNA. Displacement of the DNA strand occurs in the 3' to 5' direction with respect to the single-stranded DNA flanking the duplex. The efficiency of unwinding is found to correlate inversely with the length of the duplex region. The recombinant Sgs1 fragment is found to bind more tightly to a forked DNA substrate than to either single or double-stranded DNA. Like the DNA-DNA helicase activity, unwinding of the DNA-RNA hybrid is driven by the hydrolysis of ATP or dATP Saccharomyces cerevisiae dADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Sgs1
-
 Saccharomyces cerevisiae
Sgs1 DNA helicase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae