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Literature summary for 5.6.2.3 extracted from
- TWINKLE has 5' -> 3' DNA helicase activity and is specifically stimulated by mitochondrial single-stranded DNA-binding protein (2003), J. Biol. Chem., 278, 48627-48632 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| mitochondrial single-stranded DNA-binding protein | - |
Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 insect cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibited at low concentrations of the non-hydrolyzable ATP analogue, ATPgammaS | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 4.5 mM used in assay conditions | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-agarose column chromatography, hydroxyapatite column chromatography, and HiTrap heparin-Sepharose column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | TWINKLE is the helicase at the mitochondrial DNA replication fork with 5' to 3' directionality and distinct substrate requirements. The protein needs a stretch of 10 nucleotides of single-stranded DNA on the 5'-side of the duplex to unwind duplex DNA. In addition, helicase activity is not observed unless a short single-stranded 3'-tail is present. The helicase activity has an absolute requirement for hydrolysis of a nucleoside 5'-triphosphate, with UTP being the optimal substrate | Homo sapiens | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Homo sapiens |
| ? | x * 72000, calculated from amino acid sequence | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TWINKLE | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| CTP | very poor co-effector | Homo sapiens | |
| dTTP | - |
Homo sapiens | |
| GTP | - |
Homo sapiens | |
| UTP | best cofactor | Homo sapiens | |
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