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Literature summary for 5.6.2.3 extracted from

  • Th๖mmes, P.; Marton, R.F.; Cotterill, S.
    Purification and characterisation of a DNA helicase, dheI I, from Drosophila melanogaster embryos (1995), Nucleic Acids Res., 23, 4443-4450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
single-stranded DNA binding protein dRP-A stimulates the activity on substrates with more than 300 nucleotides double-stranded region Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
ATP substrate with optimal concentration range between 1 and 2 mM. At high concentrations inhibition of activity can be observed Drosophila melanogaster
dATP substrate with optimal concentration range between 1 and 2 mM. At high concentrations inhibition of activity can be observed Drosophila melanogaster
NaCl above 10 mM Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, optimum concentration: 0.5 mM Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration, glycerol gradient centrifugation Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O Dhel I moves 5' to 3' on the DNA strand to which it is bound. Unwinding activity decreases with increasing length of the double-stranded region suggesting a distributive mode of action. ATP and dATP are the only nucleoside-5'-triphosphates that support the strand displacement reaction. Both have an optimal concentration range between 1 and 2 mM Drosophila melanogaster ADP + phosphate
-
 ?
dATP + H2O Dhel I moves 5' to 3' on the DNA strand to which it is bound. Unwinding activity decreases with increasing length of the double-stranded region suggesting a distributive mode of action. ATP and dATP are the only nucleoside-5'-triphosphates that support the strand displacement reaction. Both have an optimal concentration range between 1 and 2 mM Drosophila melanogaster dADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
dheI I
-
 Drosophila melanogaster
DNA helicase
-
 Drosophila melanogaster