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Literature summary for 5.6.2.3 extracted from
- TWINKLE has 5' -> 3' DNA helicase activity and is specifically stimulated by mitochondrial single-stranded DNA-binding protein (2003), J. Biol. Chem., 278, 48627-48632.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| mitochondrial single-stranded DNA-binding protein | stimultes the enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATPgammaS | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Homo sapiens | TWINKLE is the helicase at the mitochondrial DNA replication fork | ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q96RR1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| generation of recombinant baculovirus encoding the human TWINKLE gene, expression in insect cells | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | TWINKLE is the helicase at the mitochondrial DNA replication fork | Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | TWINKLE is a DNA helicase with 5' to 3' directionality. The enzyme needs a stretch of 10 nucleotides of single-stranded DNA on the 5'-side of the duplex to unwind duplex DNA. In addition, helicase activity is not observed unless a short single-stranded 3'-tail is present. UTP efficiently supports DNA unwinding. ATP, GTP, and dTTP are less effective | Homo sapiens | ADP + phosphate | - |
? | |
| dTTP + H2O | TWINKLE is a DNA helicase with 5' to 3' directionality. The enzyme needs a stretch of 10 nucleotides of single-stranded DNA on the 5'-side of the duplex to unwind duplex DNA. In addition, helicase activity is not observed unless a short single-stranded 3'-tail is present. UTP efficiently supports DNA unwinding. ATP, GTP, and dTTP are less effective | Homo sapiens | dTDP + phosphate | - |
? | |
| GTP + H2O | TWINKLE is a DNA helicase with 5' to 3' directionality. The enzyme needs a stretch of 10 nucleotides of single-stranded DNA on the 5'-side of the duplex to unwind duplex DNA. In addition, helicase activity is not observed unless a short single-stranded 3'-tail is present. UTP efficiently supports DNA unwinding. ATP, GTP, and dTTP are less effective | Homo sapiens | GDP + phosphate | - |
? | |
| UTP + H2O | TWINKLE is a DNA helicase with 5' to 3' directionality. The enzyme needs a stretch of 10 nucleotides of single-stranded DNA on the 5'-side of the duplex to unwind duplex DNA. In addition, helicase activity is not observed unless a short single-stranded 3'-tail is present. UTP efficiently supports DNA unwinding. ATP, GTP, and dTTP are less effective | Homo sapiens | UDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TWINKLE | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Homo sapiens |
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