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Literature summary for 5.6.2.1 extracted from

  • Terekhova, K.; Gunn, K.H.; Marko, J.F.; Mondragon, A.
    Bacterial topoisomerase I and topoisomerase III relax supercoiled DNA via distinct pathways (2012), Nucleic Acids Res., 40, 10432-10440.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Cloned(Commentary)

Cloned (Comment) Organism
gene topA, expression in Escherichia coli strain BL21(DE3) Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene topA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information relaxation and cleavage of pBR322 DNA, differences in the mechanism of these two related type IA topoisomerases, Topo I and Topo III, overview Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Topo I
-
 Escherichia coli
Topoisomerase I
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

General Information

General Information Comment Organism
evolution activity comparison of two related type IA topoisomerases, Topo I and Topo III: Topo I initiates relaxation sooner than Topo III. and for most substrates, the pauses between relaxation runs catalyzed by Topo I are spaced by only a few seconds, whereas Topo III paused for tens of seconds in between relaxation runs Escherichia coli