Any feedback?
Literature summary for 5.6.2.1 extracted from
- Crystal structure of a bacterial topoisomerase IB in complex with DNA reveals a secondary DNA binding site (2010), Structure, 18, 725-733.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified DraTopIB enzyme in a complex with 12-bp duplex DNA, hanging drop vapor diffusion method, from 30% PEG 400 w/v, 0.1 M sodium acetate, pH 4.5, 0.2 M calcium chloride, X-ray diffraction structure determination and analysis, molecular replacement | Deinococcus radiodurans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Deinococcus radiodurans |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate is supercoiled pUC19 plasmid DNA. The enzyme contains a secondary DNA binding site located on the surface of the C-terminal domain, site architecture, overview. DNA binding mechanism and implications for TopIB-mediated DNA synapsis, modelling, detailed overview | Deinococcus radiodurans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the DraTopIB 139GSDIYARQHK148 peptide forms a well-ordered alpha-helix that mimics the specificity helix of poxvirus TopIB, comparison of structures of DraTopIB and poxvirus TopIB, overview. Several mechanisms might exist to trigger the folding of the specificity heli | Deinococcus radiodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TopIB | - |
Deinococcus radiodurans |
| topoisomerase IB | - |
Deinococcus radiodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Deinococcus radiodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Deinococcus radiodurans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme model showing that the second site capture might account for DNA crossover binding, nucleation of DNA synapsis, and plectonemic supercoiling within the synaptic filament in TopIB enzyme with both DNA sites filled, overview | Deinococcus radiodurans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
