Literature summary for 5.6.2.1 extracted from

Tuduri, S.; Crabbe, L.; Conti, C.; Tourriere, H.; Holtgreve-Grez, H.; Jauch, A.; Pantesco, V.; De Vos, J.; Thomas, A.; Theillet, C.; Pommier, Y.; Tazi, J.; Coquelle, A.; Pasero, P.
Topoisomerase I suppresses genomic instability by preventing interference between replication and transcription (2009), Nat. Cell Biol., 11, 1315-1324.

Search for more literature for 5.6.2.1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	Top1 functionally interacts with splicing factor ASF/SF2, alternative splicing factor/splicing factor 2 modulating its the function to prevent genomic instability in S phase	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phorsphorylation by Top1 kinase29 is required for activity, inhibition o by diospyrin induces a 10fold increase in DNA breaks in Top1-proficient cells	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HCT-116 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Top1 functionally interacts with splicing factor ASF/SF2, alternative splicing factor/splicing factor 2 modulating its the function to prevent genomic instability in S phase	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
Top1	-	Homo sapiens
Topoisomerase I	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	Top1-deficient cells accumulate stalled replication forks and chromosome breaks in S phase resulting in supercoiled DNA and altered RNA splicing, breaks occur preferentially at gene-rich regions of the genome	Homo sapiens
physiological function	topoisomerase I is a key enzyme in functioning at the interface between DNA replication, transcription and mRNA maturation. Top1 suppresses genomic instability in mammalian cells by preventing a conflict between transcription and DNA replication, it prevents replication fork collapse by suppressing the formation of R-loops in an ASF/SF2-dependent manner	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)