Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
K352A | site-directed mutagenesis, the mutant shows highly reduced relaxation activity compared to wild-type enzyme, the cleavage religation equilibrium is shifted towards the cleavage | Leishmania donovani |
K352R | site-directed mutagenesis, the mutant shows highly reduced relaxation activity compared to wild-type enzyme, the cleavage religation equilibrium is shifted towards the cleavage | Leishmania donovani |
R314K | site-directed mutagenesis, the mutant shows onyl slightly affected relaxation activity and only a slightly slower rate of cleavage compared to wild-type enzyme | Leishmania donovani |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DNA binding and subunit interaction analysis of wild-type and mutant enzymes, overview | Leishmania donovani | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
Topoisomerase I | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leishmania donovani |