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Literature summary for 5.6.2.1 extracted from

  • Badaracco, G.; Landsberger, N.; Benfante, R.
    Purification and characterization of a proteolytic active fragment of DNA topoisomerase I from the brine shrimp, Artemia franciscana (Crustacea anostraca) (1992), Biochem. J., 282, 249-254.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.1

Inhibitors

Inhibitors Comment Organism Structure
camptothecins
-
 Artemia franciscana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no requirement for a divalent cation, stimulation in presence of 10 mM MgCl2 Artemia franciscana
Na+ maximal activity in presence of 150 mM NaCl Artemia franciscana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 1 * 63000, SDS-PAGE. The purified 63000 MW protein is a proteolytic fragment of a protein with a molecular mass of 100000 Artemia franciscana

Organism

Organism UniProt Comment Textmining
Artemia franciscana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Artemia franciscana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Artemia franciscana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Artemia franciscana relaxed closed circular DNA
-
 ?
supercoiled DNA positive and negative supercoiled DNA Artemia franciscana relaxed closed circular DNA
-
 ?
supercoiled DNA ColE1 DNA Artemia franciscana relaxed closed circular DNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 63000, SDS-PAGE. The purified 63000 MW protein is a proteolytic fragment of a protein with a molecular mass of 100000 Artemia franciscana