Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Carboxydothermus hydrogenoformans |
Crystallization (Comment) | Organism |
---|---|
AMP-PNP-bound enzyme form, vapor batch crystallization method, using 20 mM MgCl2, 100 mM HEPES-NaOH (pH 7.5), and 22% (w/v) polyacrylic acid 5100. ADP-bound enzyme form, vapor batch crystallization method, using 200 mM NH4Cl, 10 mM MgCl2, 50 mM HEPES sodium (pH 7.0), 5% (w/v) polyethylene glycol PEG 8000, and 3% (w/v) 1,8-diaminooctane | Carboxydothermus hydrogenoformans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Carboxydothermus hydrogenoformans | - |
ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | Carboxydothermus hydrogenoformans ATCC BAA-161 | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | Q3AF10 | - |
- |
Carboxydothermus hydrogenoformans ATCC BAA-161 | Q3AF10 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, t-Butyl HIC column chromatography, and Superdex 200 gel filtration | Carboxydothermus hydrogenoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Carboxydothermus hydrogenoformans | ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | - |
Carboxydothermus hydrogenoformans ATCC BAA-161 | ADP + phosphate + an unfolded polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPN | - |
Carboxydothermus hydrogenoformans |
group III chaperonin | - |
Carboxydothermus hydrogenoformans |
General Information | Comment | Organism |
---|---|---|
metabolism | chaperonins are ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation | Carboxydothermus hydrogenoformans |