Application | Comment | Organism |
---|---|---|
medicine | the enzyme can be used as a vaccine candidate against Mycoplasma gallisepticum, a common and widespread cause of chronic respiratory disease in poultry | Mycoplasma gallisepticum |
Cloned (Comment) | Organism |
---|---|
gene groEL, DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycoplasma gallisepticum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mycoplasma gallisepticum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycoplasma gallisepticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 56915.4, mass spectrometry, x * 62000, recombinant His-tagged enzyme, SDS-PAGE | Mycoplasma gallisepticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Mycoplasma gallisepticum | - |
ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | Mycoplasma gallisepticum Rlow | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma gallisepticum | J3T995 | gene groEL | - |
Mycoplasma gallisepticum Rlow | J3T995 | gene groEL | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycoplasma gallisepticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Mycoplasma gallisepticum | ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | - |
Mycoplasma gallisepticum Rlow | ADP + phosphate + an unfolded polypeptide | - |
? | |
additional information | the recombinant enzyme possesses ATPase activity and contributes to the refolding of recombinant MG PrpC protein | Mycoplasma gallisepticum | ? | - |
? | |
additional information | the recombinant enzyme possesses ATPase activity and contributes to the refolding of recombinant MG PrpC protein | Mycoplasma gallisepticum Rlow | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56915.4, mass spectrometry, x * 62000, recombinant His-tagged enzyme, SDS-PAGE | Mycoplasma gallisepticum |
Synonyms | Comment | Organism |
---|---|---|
chaperonin GroEL | - |
Mycoplasma gallisepticum |
GroEl | - |
Mycoplasma gallisepticum |
group I chaperonin | - |
Mycoplasma gallisepticum |
Hsp60 | - |
Mycoplasma gallisepticum |
MG GroEL | - |
Mycoplasma gallisepticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mycoplasma gallisepticum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | activity range, profile overview | Mycoplasma gallisepticum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycoplasma gallisepticum | sequence calculation | - |
5.38 |
General Information | Comment | Organism |
---|---|---|
evolution | GroEL belongs to the heat shock family of proteins (HSPs), also known as Hsp60 or Group I chaperonin | Mycoplasma gallisepticum |
physiological function | the enzyme possesses ATPase activity and contributed to the refolding of MG PrpC protein, it is a protective antigen | Mycoplasma gallisepticum |