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Literature summary for 5.6.1.3 extracted from
- Alternating-site mechanism of kinesin-1 characterized by single-molecule FRET using fluorescent ATP analogues (2009), Biophys. J., 97, 173-182.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(RIL) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0134 | - |
ATP | +/-0.0006 mM, Vmax: 575 nmol/s, pH and temperature not specified in the publication | Homo sapiens |  |
| 0.032 | - |
Alexa Fluor 647 ATP | +/-0.022 mM, Vmax: 247 nmol/s, pH and temperature not specified in the publication. The fluorescent ATP analog can fuel kinesin-1'S processsive motion and is a suitable substrate analog, but it alters the motor's kinetic parameters | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
a 391 amino acid long version of human ubiquitous kinesin KIF5B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by phosphocellulose an Q-Sepharose chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Alexa Fluor 647 ATP + H2O + a kinesin associated with a microtubule at position n | Alexa Fluor 647 ATP, a fluorescent ATP analog, activity determined by FRET-based assays, Förster resonance energy transfer assays. The fluorescent ATP analog can fuel kinesin-1'S processsive motion and is a suitable substrate analog, but it alters the motor's kinetic parameters | Homo sapiens | Alexa Fluor 647 ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| ATP + H2O + a kinesin associated with a microtubule at position n | - |
Homo sapiens | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| kinesin-1 | a 391 amino acid long version of human ubiquitous kinesin KIF5B | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | kinesin-1 motor proteins move along microtubules in reptitive steps of 8 nm at the expense of ATP | Homo sapiens |
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