Literature summary for 5.6.1.3 extracted from

Grissom, P.M.; Fiedler, T.; Grishchuk, E.L.; Nicastro, D.; West, R.R.; McIntosh, J.R.
Kinesin-8 from fission yeast: a heterodimeric, plus-end-directed motor that can couple microtubule depolymerization to cargo movement (2009), Mol. Biol. Cell, 20, 963-972.

Search for more literature for 5.6.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
microtubule	-	Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3)pLysS cells or Sf9 cells, overexpression of Klp5p or Klp6p in fission yeast is lethal	Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	ATP	-	Schizosaccharomyces pombe

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
118000	-	Klp6p	Schizosaccharomyces pombe
184000	-	Klp5p	Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Schizosaccharomyces pombe	kinesin-8 contributes both to chromosome congression to the metaphase plate and to the coupling of spindle microtubules to kinetochores during anaphase A	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Superdex 200 gel filtration, and SP Sepharose column chromatography	Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	kinesin-8 expressed in insect cells forms a heterodimer that hydrolyzes ATP in a microtubule-dependent manner and moves on microtubules toward their plus ends	Schizosaccharomyces pombe	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?
additional information	kinesin-8 contributes both to chromosome congression to the metaphase plate and to the coupling of spindle microtubules to kinetochores during anaphase A	Schizosaccharomyces pombe	?	-	?

Subunits

Subunits	Comment	Organism
heterodimer	x-ray crystallography	Schizosaccharomyces pombe

Synonyms

Synonyms	Comment	Organism
kinesin-8	plus-end-directed motor	Schizosaccharomyces pombe
Klp5p	plus-end-directed motor	Schizosaccharomyces pombe
Klp6p	the motor-plus-neck domain of Klp6p forms soluble dimers that cross-link microtubules and show both microtubule-activated ATPase and plus-end-directed motor activities	Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.4	-	ATP	-	Schizosaccharomyces pombe

pI Value

Organism	Comment	pI Value Maximum	pI Value
Schizosaccharomyces pombe	theoretical isoelectric point of Klp5p	-	5.6
Schizosaccharomyces pombe	theoretical isoelectric point of Klp6p	-	10.6

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Release 2023.1 (January 2023)