Inhibitors | Comment | Organism | Structure |
---|---|---|---|
monastrol | model for dimeric Eg5 in which monastrol stabilizes a conformation with the neck linker of each motor domain locked onto its catalytic core, resulting in dissociation of the MT. Eg5 complex or an inhibition of MT binding | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
ATP | dimeric Eg5-513 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | Homo sapiens | - |
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Homo sapiens | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
ATP + H2O + a kinesin associated with a microtubule at position n | Eg5 is a homotetrameric kinesin-5 involved in centrosome separation and assembly of the bipolar mitotic spindle. The stable dimer Eg5-513 promotes robust plus-end-directed microtubule gliding at a rate similar to that of homotetrameric Eg5 in vitro. Eg5-513 exhibits slow ATP turnover, high affinity for ATP, and a weakened affinity for microtubules when compared to monomeric Eg5 | Homo sapiens | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Eg5 is a homotetrameric kinesin-5 involved in centrosome separation and assembly of the bipolar mitotic spindle. The stable dimer Eg5-513 promotes robust plus-end-directed microtubule gliding at a rate similar to that of homotetrameric Eg5 in vitro. Eg5-513 exhibits slow ATP turnover, high affinity for ATP, and a weakened affinity for microtubules when compared to monomeric Eg5 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
kinesin Eg5 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
ATP | dimeric Eg5-513 | Homo sapiens |