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Literature summary for 5.6.1.3 extracted from

  • Cochran, J.C.; Krzysiak, T.C.; Gilbert, S.P.
    Pathway of ATP hydrolysis by monomeric kinesin Eg5 (2006), Biochemistry, 45, 12334-12344.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of truncated kiesin Eg5-367 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 ATP monomeric kinesin Eg5 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n Homo sapiens monomeric kinesin Eg5 has a motor tightly bound to the microtubule during a majority of its ATPase cycle to generate and sustain force in the mitotic spindle ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens
-
 isoform Eg5/KSP
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) the microtubule-Eg5 complex binds MgATP tightly, followed by rapid ATP hydrolysis with a subsequent slow step that limits steady-state turnover. Presence of microtubules accelerates the kinetics of each step in the ATPase pathway and amplifies the nucleotide-dependent structural transitions required for force generation. Phosphate product release and Eg5 detachment from the microtubule are coupled with a step occuring at the slow rate after ATP hydrolysis followed by the second step occuring more rapidly Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n monomeric kinesin Eg5 has a motor tightly bound to the microtubule during a majority of its ATPase cycle to generate and sustain force in the mitotic spindle Homo sapiens ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n the microtubule-Eg5 complex binds MgATP tightly, followed by rapid ATP hydrolysis with a subsequent slow step that limits steady-state turnover. Presence of microtubules accelerates the kinetics of each step in the ATPase pathway and amplifies the nucleotide-dependent structural transitions required for force generation. Phosphate product release and Eg5 detachment from the microtubule are coupled with a step occuring at the slow rate after ATP hydrolysis followed by the second step occuring more rapidly Homo sapiens ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Synonyms

Synonyms Comment Organism
kinesin Eg5
-
 Homo sapiens
KSP
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
 ATP
-
 Homo sapiens