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Literature summary for 5.5.1.11 extracted from

  • Kuhm, A.E.; Schloemann, M.; Knackmuss, H.J.; Pieper, D.H.
    Purification and characterization of dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134 (1990), Biochem. J., 266, 877-883.
    View publication on PubMedView publication on EuropePMC
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Inhibitors

Inhibitors Comment Organism Structure
citrate
-
 Cupriavidus necator
EDTA 1.3 mM Cupriavidus necator
PCMB
-
 Cupriavidus necator
phosphate
-
 Cupriavidus necator
succinate
-
 Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator
0.128
-
 2-methyl-cis,cis-muconate
-
 Cupriavidus necator
0.158
-
 3-methyl-cis,cis-muconate
-
 Cupriavidus necator
0.304
-
 3-chloro-cis,cis-muconate
-
 Cupriavidus necator
0.502
-
 2-chloro-cis,cis-muconate
-
 Cupriavidus necator
4.2
-
 cis,cis-muconate
-
 Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ requirement Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 6 * 40000, SDS-PAGE Cupriavidus necator
270000
-
 gel filtration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
 JMP 134
-
Cupriavidus necator JMP 134-1
-
 JMP 134
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:2,4-dichlorophenoxyacetic acid-grown cell
-
 Cupriavidus necator
-
culture condition:benzoate-grown cell
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80.4
-
-
 Cupriavidus necator

Storage Stability

Storage Stability Organism
-16°C, without MnSO4, 95% loss of activity after 10 days Cupriavidus necator
4°C, pH 7.5-8.5, 10-30% loss of activity after 24 h Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator 4-carboxymethylene-3-chlorobut-2-en-4-olide + HCl
-
 ?
2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 4-carboxymethylene-3-chlorobut-2-en-4-olide + HCl
-
 ?
2-Chloro-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
2-Chloro-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
2-Methyl-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
2-Methyl-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
3-chloro-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
3-Methyl-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
3-Methyl-cis,cis-muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
cis,cis-Muconate
-
 Cupriavidus necator ?
-
 ?
cis,cis-Muconate
-
 Cupriavidus necator JMP 134-1 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 40000, SDS-PAGE Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.2
-
 2-methyl-cis,cis-muconate
-
 Cupriavidus necator
15.8
-
 2-chloro-cis,cis-muconate
-
 Cupriavidus necator
51.5
-
 cis,cis-muconate
-
 Cupriavidus necator
54.8
-
 3-methyl-cis,cis-muconate
-
 Cupriavidus necator
63.7
-
 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator
119
-
 3-chloro-cis,cis-muconate
-
 Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 above 80% of activity maximum at pH 5.5 and 8 Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
additional information thiol groups required Cupriavidus necator