Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains one atom of zinc per 63000 Da monomer. This zinc ion could not be removed by treatment with EDTA or urea. However, a zinc-depleted enzyme is obtained after prolonged dialysis against the specific chelating agent 1,10-phenanthroline. Removal of the zinc ion results in inactivation of the enzyme with concomitant loss of its ability to bind tRNA. Structural role for the zinc in the tRNA-pseudouridine synthetase Pus1 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12211 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PUS1 | - |
Saccharomyces cerevisiae |