Protein Variants | Comment | Organism |
---|---|---|
Q450H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme produng exclusively achilleol A as product | Saccharomyces cerevisiae |
Q450H/V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol, and protosta-16,24-dien-3beta-ol | Saccharomyces cerevisiae |
T384Y | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing lanosterol, parkeol, and 9beta-lanosta-7,24-dien-3beta-ol | Saccharomyces cerevisiae |
T384Y/Q450H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly parkeol and very low amounts of 9beta-lanosta-7,24-dien-3beta-ol and lanosterol | Saccharomyces cerevisiae |
T384Y/Q450H/V454I | site-directed mutagenesis, the mutant produces exclusively reaction intermediate parkeol but not lanosterol as the sole end product | Saccharomyces cerevisiae |
T384Y/V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly parkeol and lower amounts of 9beta-lanosta-7,24-dien-3beta-ol and lanosterol | Saccharomyces cerevisiae |
V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly lanosterol and a low amount of (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of wild-type enzyme | Saccharomyces cerevisiae | lanosterol | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant Q450H | Saccharomyces cerevisiae | achilleol A | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant V454I | Saccharomyces cerevisiae | lanosterol + (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant Q450H/V454I | Saccharomyces cerevisiae | lanosterol + (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol + protosta-16,24-dien-3beta-ol | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutants T384Y, T384Y/Q450H, and T384Y/V454I | Saccharomyces cerevisiae | lanosterol + parkeol + 9beta-lanosta-7,24-dien-3beta-ol | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant T384Y/Q450H/V454I | Saccharomyces cerevisiae | parkeol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ERG7 | - |
Saccharomyces cerevisiae |
OSC | - |
Saccharomyces cerevisiae |
Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |