Protein Variants | Comment | Organism |
---|---|---|
F699H | the mutant produces lanosterol (13%), protosta-13(17)-dien-3beta-ol (70%), and (17Z)-protosta-17(20),24-dien-3beta-ol (17%) | Saccharomyces cerevisiae |
F699I | the mutant produces lanosterol (100%) as the wild type enzyme | Saccharomyces cerevisiae |
F699L | the mutant produces lanosterol (100%) as the wild type enzyme | Saccharomyces cerevisiae |
F699M | the mutant produces lanosterol (13%), (17Z)-protosta-17(20),24-dien-3beta-ol (46%), protosta-13(17)-dien-3beta-ol (70%), malabarica-14E,17E,21-trien-3beta-ol (7%), 17alpha-protosta-20,24-dien-3beta-ol (1%), (17Z)-protosta-17(20),24-dien-3beta-ol (10%), (13alphaH)-isomalabarica-14E,17E,21-dien-3 beta-ol (17%), and (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol (18%) | Saccharomyces cerevisiae |
F699N | nonviable mutant, does not produce lanosterol, but protosta-13(17)-dien-3beta-ol (55%), malabarica-14E,17E,21-trien-3beta-ol (5%), 17alpha-protosta-20,24-dien-3beta-ol (24%), and (17Z)-protosta-17(20),24-dien-3beta-ol (16%) | Saccharomyces cerevisiae |
F699P | the mutant produces lanosterol (100%) as the wild type enzyme | Saccharomyces cerevisiae |
F699T | the mutant produces lanosterol (less than 0.2%) and protosta-13(17)-dien-3beta-ol (above 99.8%) | Saccharomyces cerevisiae |
F699X | TKW14[pERG7F699X] site-saturated mutants allow for ergosterol-independent growth, with the exception of Leu, Ile, His, Met, Pro, and Thr substitutions | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae TKW14 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
(3S)-2,3-oxidosqualene | - |
Saccharomyces cerevisiae TKW14 | lanosterol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ERG7 | - |
Saccharomyces cerevisiae |
OSC | - |
Saccharomyces cerevisiae |
Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |