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Literature summary for 5.4.99.5 extracted from

  • Li, P.P.; Liu, Y.J.; Liu, S.J.
    Genetic and biochemical identification of the chorismate mutase from Corynebacterium glutamicum (2009), Microbiology, 155, 3382-3391.
    View publication on PubMed
Search for more literature for 5.4.99.5

Activating Compound

Activating Compound Comment Organism Structure
additional information the CM0819 activity is not affected by L-Phe, L-Tyr or L-Trp (0.01-10 mM), EDTA at concentrations of 0.1-2 mM does not affect CM0819 activity Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 2 mM Co2+ decreases CM0819 activity by 52.4% Corynebacterium glutamicum
Mn2+ 2 mM Mn2+ decreases CM0819 activity by 38.9% Corynebacterium glutamicum
Ni2+ 2 mM Ni2+ decreases CM0819 activity by 41.8% Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.83
-
 chorismate at 37°C Corynebacterium glutamicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Corynebacterium glutamicum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ at concentrations of 0.1-2 mM does not affect CM0819 activity Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11300
-
 subunit, calculated from amino acid sequence Corynebacterium glutamicum
12000
-
 2 * 12000, SDS-Page Corynebacterium glutamicum
23500
-
 gel filtration Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
 strain ATCC 13032
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind protein purification column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Corynebacterium glutamicum Prephenate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 12000, SDS-Page Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
CM0819
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.05
-
 chorismate at 37°C Corynebacterium glutamicum

General Information

General Information Comment Organism
metabolism chorismate mutase is the first and the key enzyme that diverges the shikimate pathway to either tryptophan or phenylalanine and tyrosine Corynebacterium glutamicum
physiological function CM0819 significantly stimulates 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DS2098) activity, CM0819 interacts with 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DS2098 from Corynebacterium glutamicum and this interaction results in allosteric regulation of DS2098 synthase activity Corynebacterium glutamicum