Any feedback?
Literature summary for 5.4.99.5 extracted from
- A small, thermostable, and monofunctional chorismate mutase from the archaeon Methanococcus jannaschii (1998), Biochemistry, 37, 10062-10073.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
| expression in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.041 | - |
chorismate | 30°C | Methanocaldococcus jannaschii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25790 | - |
gel filtration | Escherichia coli |
| 29390 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Escherichia coli | biosynthesis of aromatic amino acids | Prephenate | - |
? | |
| Chorismate | Methanocaldococcus jannaschii | biosynthesis of aromatic amino acids | Prephenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A9J8 | - |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
| N-terminal Met1 is in most samples removed | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| misfolded, higher-order aggregates convert into fully active dimeric protein by denaturation with guanidinium chloride and subsequent renaturation upon dilution into native buffer | Escherichia coli |
| refolding after treatment with guanidinium chloride | Methanocaldococcus jannaschii |
| refolding after treatment with urea | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Escherichia coli | Prephenate | - |
? | |
| Chorismate | - |
Methanocaldococcus jannaschii | Prephenate | - |
? | |
| Chorismate | biosynthesis of aromatic amino acids | Escherichia coli | Prephenate | - |
? | |
| Chorismate | biosynthesis of aromatic amino acids | Methanocaldococcus jannaschii | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, 2 * 12843-12848, deduced from amino acid sequence, ESI-MS, MALDI-MS | Methanocaldococcus jannaschii |
| homodimer | alpha2, 2 * 11883-11887, deduced from amino acid sequence, ESI-MS, MALDI-MS | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase | - |
Methanocaldococcus jannaschii |
| chorismate mutase | - |
Escherichia coli |
| EcCM | - |
Escherichia coli |
| MjCM | - |
Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 63 | - |
midpoint of unfolding transitions | Escherichia coli |
| 88 | - |
midpoint of unfolding transitions | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.7 | - |
chorismate | 30°C | Methanocaldococcus jannaschii | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Methanocaldococcus jannaschii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
