Cloned (Comment) | Organism |
---|---|
gene bAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Saccharomyces cerevisiae strain GIL77, real-time RT-PCR enzyme expression analysis, MS analysis metabolite identification | Platycodon grandiflorus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | Platycodon grandiflorus | - |
beta-amyrin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Platycodon grandiflorus | A0A1Z2WUZ5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Platycodon grandiflorus | - |
leaf | - |
Platycodon grandiflorus | - |
additional information | about equal enzyme expression in the different tissues, quantitative tissue-specific real-time RT-PCR gene expression analysis | Platycodon grandiflorus | - |
root | - |
Platycodon grandiflorus | - |
stem | - |
Platycodon grandiflorus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Platycodon grandiflorus | beta-amyrin | - |
r | |
additional information | GC-MS analysis metabolite identification | Platycodon grandiflorus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 87996, sequence calculation | Platycodon grandiflorus |
Synonyms | Comment | Organism |
---|---|---|
bAS | - |
Platycodon grandiflorus |
PlgOSC1 | - |
Platycodon grandiflorus |
General Information | Comment | Organism |
---|---|---|
evolution | PlgOSC1 not only contains a DCTAE motif that is implicated in substrate binding, but also has four repeats of the QXXXGXW motif, which is a typical feature of the triterpene synthase superfamily. PlgOSC1 has a Try residue in the MWCYCR motif that plays a role in the formation of beta-amyrin | Platycodon grandiflorus |
metabolism | the enzyme synthesizes beta-amyrin, a central precursor in the platycoside biosynthesis pathway, overview. 69 platycosides are identified from Platycodon grandiflorum | Platycodon grandiflorus |