BRENDA - Enzyme Database show
show all sequences of 5.4.99.33

Cucurbitadienol synthase, the first committed enzyme for cucurbitacin biosynthesis, is a distinct enzyme from cycloartenol synthase for phytosterol biosynthesis

Shibuya, M.; Adachi, S.; Ebizuka, Y.; Tetrahedron 60, 6995-7003 (2004)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in lanosterol synthase deficient (erg7) Saccharomyces cerevisiae strain GIL77
Cucurbita pepo
Engineering
Amino acid exchange
Commentary
Organism
I489V
the mutation does not result in the change of product-specificity
Cucurbita pepo
L488P
the mutation does not result in the change of product-specificity
Cucurbita pepo
LI488/489PV
the mutation does not result in the change of product-specificity
Cucurbita pepo
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cucurbita pepo
Q6BE24
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Cucurbita pepo
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-squalene-2,3-epoxide
-
716949
Cucurbita pepo
cucurbitadienol
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in lanosterol synthase deficient (erg7) Saccharomyces cerevisiae strain GIL77
Cucurbita pepo
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
I489V
the mutation does not result in the change of product-specificity
Cucurbita pepo
L488P
the mutation does not result in the change of product-specificity
Cucurbita pepo
LI488/489PV
the mutation does not result in the change of product-specificity
Cucurbita pepo
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Cucurbita pepo
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-squalene-2,3-epoxide
-
716949
Cucurbita pepo
cucurbitadienol
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
cucurbitadienol synthase, the first committed enzyme for cucurbitacin biosynthesis, is a distinct enzyme from cycloartenol synthase for phytosterol biosynthesis
Cucurbita pepo
General Information (protein specific)
General Information
Commentary
Organism
metabolism
cucurbitadienol synthase, the first committed enzyme for cucurbitacin biosynthesis, is a distinct enzyme from cycloartenol synthase for phytosterol biosynthesis
Cucurbita pepo
Other publictions for EC 5.4.99.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746042
Zhang
Oxidation of cucurbitadienol ...
Siraitia grosvenorii
Plant Cell Physiol.
57
1000-1007
2016
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1
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2
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746038
Dai
Functional characterization o ...
Siraitia grosvenorii
Plant Cell Physiol.
56
1172-1182
2015
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749428
Davidovich-Rikanati
Recombinant yeast as a functi ...
Citrullus colocynthis, Cucurbita pepo, no activity in Citrullus lanatus var. lanatus
Yeast
32
103-114
2015
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2
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9
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716949
Shibuya
-
Cucurbitadienol synthase, the ...
Cucurbita pepo
Tetrahedron
60
6995-7003
2004
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