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Literature summary for 5.4.99.11 extracted from
- Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae (2011), Biores. Technol., 102, 9179-9184.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and expression in Escherichia coli strain DH10B, expression on cell surface of Saccharomyces cerevisiae strain FMB-1 | Enterobacter sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. strain FMB-1 on Saccharomyces cerevisiae strain EBY100, stable at a broad range of temperatures (35-55°C) and pHs (pH 5-7) with optimal temperature and pH at 45°C and pH 7.0, respectively | Enterobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter sp. | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sucrose isomerase | - |
Enterobacter sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant enzyme displayed on Saccharomyces cerevisiae surface and expressed in Escherichia coli strain DH10B | Enterobacter sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | recombinant enzyme displayed on Saccharomyces cerevisiae surface shows higher activity than the enzyme expressed in Escherichia coli strain DH10B | Enterobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 55 | recombinant enzyme displayed on Saccharomyces cerevisiae surface, stable at, half-life at 50°C is 2.4 h | Enterobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme expressed in Escherichia coli strain DH10B | Enterobacter sp. |
| 7 | - |
recombinant enzyme displayed on Saccharomyces cerevisiae surface | Enterobacter sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | recombinant enzyme displayed on Saccharomyces cerevisiae surface, stable at | Enterobacter sp. |
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