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Literature summary for 5.4.4.2 extracted from

  • Su, H.; Song, S.; Yan, X.; Fang, L.; Zeng, B.; Zhu, Y.
    Endogenous salicylic acid shows different correlation with baicalin and baicalein in the medicinal plant Scutellaria baicalensis Georgi subjected to stress and exogenous salicylic acid (2018), PLoS ONE, 13, e0192114 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.4.2

Cloned(Commentary)

Cloned (Comment) Organism
gene ICS, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR enzyme expression analysis Scutellaria baicalensis

Organism

Organism UniProt Comment Textmining
Scutellaria baicalensis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Scutellaria baicalensis
-
inflorescence
-
 Scutellaria baicalensis
-

Synonyms

Synonyms Comment Organism
ICS
-
 Scutellaria baicalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Scutellaria baicalensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Scutellaria baicalensis

Expression

Organism Comment Expression
Scutellaria baicalensis expression of ICS is downregulated significantly by 67% under drought stress. ICS gene expression is upregulated after 0.140 mM salicylate treatment, but is downregulated following treatment with 0.070 or 0.280 mM salicylate. Treatment with 0.140 mM salicylate results in 1.81, 1.04, and 2.41fold increased expression at 24, 48, and 72 h, respectively. The lowest expression level in the 0.070 mM treatment is detected at 24 h and corresponds to a decrease of 99.4%, while the lowest level in the 0.280 mM is observed at 72 h and corresponds to a 66% decrease. A non-significant 1.04fold increase at 48 h constitutes the lowest expression level in the 0.140 mM treatment down
Scutellaria baicalensis expression of ICS is significantly upregulated by 38-53% under salt stress. ICS gene expression is upregulated after 0.140 mM salicylate treatment, but is downregulated following treatment with 0.070 or 0.280 mM salicylate. Treatment with 0.140 mM salicylate results in 1.81, 1.04, and 2.41fold increased expression at 24, 48, and 72 h, respectively. The lowest expression level in the 0.070 mM treatment is detected at 24 h and corresponds to a decrease of 99.4%, while the lowest level in the 0.280 mM is observed at 72 h and corresponds to a 66% decrease. A non-significant 1.04fold increase at 48 h constitutes the lowest expression level in the 0.140 mM treatment up

General Information

General Information Comment Organism
evolution the predicted ICS protein has high amino acid sequence identity to its orthologues and possesses a conserved chorismate binding site belonging to the supergene family of chorismate binding proteins Scutellaria baicalensis
metabolism salicylic acid is synthesized via the phenylalanine lyase (PAL) and isochorismate synthase (ICS) pathways and can influence the stress response in plants by regulating certain secondary metabolites. Both free salicylate and total salicylate are positively correlated with PAL, ICS, and baicalin, but negatively correlated with baicalein, overview Scutellaria baicalensis