Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0415 | - |
chorismate | pH 7.0, 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | stromal protein | Arabidopsis thaliana | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7H8 | activity is not regulated by light-dependent changes in stromal pH value, Mg2+ or redox potential | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | precursor protein of 62000 Da is cleaved to 58000 Da upon import into plastid | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.241 | - |
pH 7.0, 30°C, recombinant protein | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Arabidopsis thaliana | Isochorismate | reaction operates near equilibrium, equilibrium constant is 0.89. Enzyme does not convert chorismate directly to salicylic acid | r | |
isochorismate | - |
Arabidopsis thaliana | chorismate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 37 | more than 90% of maximum activity within this range | Arabidopsis thaliana |
4 | 44 | more than 75% of maximum activity within this range | Arabidopsis thaliana |
70 | - |
no activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.645 | - |
chorismate | pH 7.0, 30°C | Arabidopsis thaliana | |
6.08 | - |
chorismate | pH 7.0, 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Arabidopsis thaliana |