Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutamate | 3fold activation of tagless recombinant enzyme | Brassica napus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45500 | - |
2 * 45500, SDS-PAGE, native and recombinant GSA-AT | Brassica napus |
88000 | - |
native and recombinant GSA-AT, gel filtration | Brassica napus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-amino-5-oxopentanoate | Brassica napus | involved in chlorophyll biosynthesis | 5-aminolevulinate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GSA-AT | Brassica napus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
recombinant GSA-AT | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-amino-5-oxopentanoate | - |
Brassica napus | 5-aminolevulinate | - |
r | |
(S)-4-amino-5-oxopentanoate | involved in chlorophyll biosynthesis | Brassica napus | 5-aminolevulinate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45500, SDS-PAGE, native and recombinant GSA-AT | Brassica napus |
Synonyms | Comment | Organism |
---|---|---|
GSA-AT | - |
Brassica napus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Brassica napus |