Protein Variants | Comment | Organism |
---|---|---|
D165N | about 0.1% of wild-type activity, 0.23 mol iron and 0.13 mol sulfur per mol of subunit | Clostridium subterminale |
D172N | no catalytic activity, 0.43 mol iron and 0.13 mol sulfur per mol of subunit | Clostridium subterminale |
D293N | no catalytic activity, 0.97 mol iron and 0.05 mol sulfur per mol of subunit. Involved in binding of epsilon-aminium and alpha-carboxylate groups of substrate | Clostridium subterminale |
D330A | no catalytic activity, 1.9 mol iron and 1.3 mol sulfur per mol of subunit. Involved in binding of epsilon-aminium and alpha-carboxylate groups of substrate | Clostridium subterminale |
D330N | no catalytic activity, 1.0 mol iron and 0.63 mol sulfur per mol of subunit | Clostridium subterminale |
D96N | less than 1% of wild-type activity, 1.2 mol iron and 0.95 mol sulfur per mol of subunit | Clostridium subterminale |
E236Q | about 1% of wild-type activity, 0.7 mol iron and 0.5 mol sulfur per mol of subunit | Clostridium subterminale |
E86Q | about 20% of wild-type activity, 0.5 mol iron and 0.4 mol sulfur per mol of subunit | Clostridium subterminale |
R130K | no catalytic activity, 0.2 mol iron and 0.3 mol sulfur per mol of subunit | Clostridium subterminale |
R130Q | no catalytic activity, 0.42 mol iron and 0.2 mol sulfur per mol of subunit | Clostridium subterminale |
R134K | no catalytic activity, 1.5 mol iron and 1.5 mol sulfur per mol of subunit | Clostridium subterminale |
R134Q | no catalytic activity, 2.8 mol iron and 2.7 mol sulfur per mol of subunit. Involved in binding of epsilon-aminium and alpha-carboxylate groups of substrate | Clostridium subterminale |
R135K | about 16% of wild-type activity, 2.4 mol iron and 1.6 mol sulfur per mol of subunit | Clostridium subterminale |
R135Q | about 1.5% of wild-type activity, 1.8 mol iron and 1.5 mol sulfur per mol of subunit | Clostridium subterminale |
R136Q | about 1.2% of wild-type activity, 0.48 mol iron and 0.35 mol sulfur per mol of subunit | Clostridium subterminale |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | [4Fe-4S] cluster, 2.7 mol of iron per mol of subunit | Clostridium subterminale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium subterminale | Q9XBQ8 | - |
- |
Clostridium subterminale SB4 | Q9XBQ8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
recombinant wild-type with His-tag | Clostridium subterminale |
64 | - |
wild-type | Clostridium subterminale |
88 | - |
wild-type, Fe-S centers reconstituted | Clostridium subterminale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Clostridium subterminale | (3S)-3,6-diaminohexanoate | - |
? | |
L-lysine | - |
Clostridium subterminale SB4 | (3S)-3,6-diaminohexanoate | - |
? |