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Literature summary for 5.4.2.12 extracted from
- Molecular, biochemical characterization and assessment of immunogenic potential of cofactor-independent phosphoglycerate mutase against Leishmania donovani A step towards exploring novel vaccine candidate (2018), Parasitology, 145, 508-526 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme is a potential vaccine candidate against visceral leishmaniasis | Leishmania donovani |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon Plus (DE3) cells | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SPB00184 | i.e. 2-hydroxyiminoindane-1,3-dione | Leishmania donovani |  |
| TL00638 | i.e. 1-methyl-1H-imidazole-4,5-dicarboxylic acid | Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.143 | - |
2-phospho-D-glycerate | at pH 7.6 and 37°C | Leishmania donovani | |
| 0.302 | - |
3-phospho-D-glycerate | at pH 7.6 and 37°C | Leishmania donovani | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Leishmania donovani | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67 | - |
gel filtration | Leishmania donovani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | Leishmania donovani | - |
3-phospho-D-glycerate | - |
r | |
| 3-phospho-D-glycerate | Leishmania donovani | - |
2-phospho-D-glycerate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | D7R810 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Leishmania donovani | 3-phospho-D-glycerate | - |
r | |
| 3-phospho-D-glycerate | - |
Leishmania donovani | 2-phospho-D-glycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cofactor-independent phosphoglycerate mutase | - |
Leishmania donovani |
| iPGAM | - |
Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is independent from 2,3-bisphosphoglycerate | Leishmania donovani |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0373 | - |
at pH 7.6 and 37°C | Leishmania donovani | TL00638 | |
| 0.0609 | - |
at pH 7.6 and 37°C | Leishmania donovani | SPB00184 | |
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