BRENDA - Enzyme Database
show all sequences of 5.3.3.3

The enzymatic isomerization of beta-methylvinylacetyl coenzyme A and the specificity of a bacterial coenzyme A carboxylase

Rilling, H.C.; Coon, M.J.; J. Biol. Chem. 235, 3087-3092 (1960)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
beta-methylcrotonyl-CoA
-
Bos taurus
additional information
not inhibited by iodoacetate
Bos taurus
N-ethylmaleimide
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
vinylacetyl-CoA
beta-methylvinylacetyl-CoA
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
not influenced by Na+ or K+
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-
Pseudomonas sp.
-
-
-
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
-
Sus scrofa
-
kidney
-
Sus scrofa
-
liver
-
Bos taurus
-
liver
-
Gallus gallus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
32
-
substrate beta-vinylacetyl-CoA
Bos taurus
Storage Stability
Storage Stability
Organism
frozen or at 5°C, stable for months
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-Hexenoyl-CoA
-
2960
Bos taurus
?
-
-
-
-
Beta-Methylvinylacetyl-CoA
ir
2960
Bos taurus
Beta-Methylvinylacetyl-CoA
-
2960
Bos taurus
-
additional information
not 4-pentenoyl-CoA, beta-methylvinylacetate, beta-vinylacetyl ethyl ester, 5-androstan-3,17-dione, 5-cholestenone, 5-pregnene-3,20-dione
2960
Bos taurus
?
-
-
-
-
Vinylacetyl pantetheine
-
2960
Bos taurus
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
beta-methylcrotonyl-CoA
-
Bos taurus
additional information
not inhibited by iodoacetate
Bos taurus
N-ethylmaleimide
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
vinylacetyl-CoA
beta-methylvinylacetyl-CoA
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
not influenced by Na+ or K+
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
-
Sus scrofa
-
kidney
-
Sus scrofa
-
liver
-
Bos taurus
-
liver
-
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
32
-
substrate beta-vinylacetyl-CoA
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
frozen or at 5°C, stable for months
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-Hexenoyl-CoA
-
2960
Bos taurus
?
-
-
-
-
Beta-Methylvinylacetyl-CoA
ir
2960
Bos taurus
Beta-Methylvinylacetyl-CoA
-
2960
Bos taurus
-
additional information
not 4-pentenoyl-CoA, beta-methylvinylacetate, beta-vinylacetyl ethyl ester, 5-androstan-3,17-dione, 5-cholestenone, 5-pregnene-3,20-dione
2960
Bos taurus
?
-
-
-
-
Vinylacetyl pantetheine
-
2960
Bos taurus
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Bos taurus
Other publictions for EC 5.3.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
2964
Puoilly
The enzymes of beta-oxidation ...
Rhodococcus ruber
Biochem. Soc. Trans.
22
223S
1994
-
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-
1
-
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-
-
-
-
-
2965
Scherf
Succinate-ethanol fermentation ...
Clostridium kluyveri
Arch. Microbiol.
161
239-245
1994
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-
-
-
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1
2
-
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1
1
-
1
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1
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1
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1
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1
2
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1
-
1
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1
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-
2966
Scherf
Purification and properties of ...
Clostridium aminobutyricum
Eur. J. Biochem.
215
421-429
1993
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2
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2
2
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2
1
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1
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1
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1
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1
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2
-
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2
2
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-
1
-
1
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1
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2967
Yamamoto
Investigation of peroxisomal l ...
Cavia porcellus
J. Histochem. Cytochem.
40
1909-1918
1992
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1
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1
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1
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1
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1
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2213
Imamura
Purification of the multienzym ...
Pseudomonas fragi
J. Biochem.
107
184-189
1990
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1
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1
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1
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1
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1
1
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-
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-
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-
1
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1
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1
-
1
1
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2209
Pramanik
Multienzyme complexes of fatty ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochim. Biophys. Acta
750
41-46
1983
-
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177
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2
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2
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2
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2
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-
2962
Binstock
Fatty acid oxidation complex f ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
71
403-411
1981
-
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-
-
-
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-
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177
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1
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1
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2961
Schleicher
On the mechanism and some prop ...
Clostridium kluyveri
Hoppe-Seyler's Z. Physiol. Chem.
357
535-541
1976
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1
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2
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1
1
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2
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2
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2960
Rilling
The enzymatic isomerization of ...
Bos taurus, Gallus gallus, Pseudomonas sp., Sus scrofa
J. Biol. Chem.
235
3087-3092
1960
-
-
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4
1
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1
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4
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1
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4
1
1
4
-
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1
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1
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1
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4
-
1
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1
-
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1
-
4
1
1
4
-
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1
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