BRENDA - Enzyme Database
show all sequences of 5.3.3.14

A new mechanism for anaerobic unsaturated fatty acid formation in Streptococcus pneumoniae

Marrakchi, H.; Choi, K.H.; Rock, C.O.; J. Biol. Chem. 277, 44809-44816 (2002)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
4 * 31000, SDS-PAGE
Streptococcus pneumoniae
129000
-
gel filtration
Streptococcus pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
direct assay for enzyme activity based on substrate analogues
Streptococcus pneumoniae
0.000087
-
pH 7.0
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme does not catalyze dehydration reaction of EC 4.2.1.60
665480
Streptococcus pneumoniae
?
-
-
-
-
trans-2-decenoyl-N-acetylcysteamine
-
665480
Streptococcus pneumoniae
cis-3-decenoyl-N-acetylcysteamine
-
-
-
?
trans-2-octenoyl-N-acetylcysteamine
-
665480
Streptococcus pneumoniae
cis-3-octenoyl-N-acetylcysteamine
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 31000, SDS-PAGE
Streptococcus pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
4 * 31000, SDS-PAGE
Streptococcus pneumoniae
129000
-
gel filtration
Streptococcus pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
direct assay for enzyme activity based on substrate analogues
Streptococcus pneumoniae
0.000087
-
pH 7.0
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme does not catalyze dehydration reaction of EC 4.2.1.60
665480
Streptococcus pneumoniae
?
-
-
-
-
trans-2-decenoyl-N-acetylcysteamine
-
665480
Streptococcus pneumoniae
cis-3-decenoyl-N-acetylcysteamine
-
-
-
?
trans-2-octenoyl-N-acetylcysteamine
-
665480
Streptococcus pneumoniae
cis-3-octenoyl-N-acetylcysteamine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 31000, SDS-PAGE
Streptococcus pneumoniae
Other publictions for EC 5.3.3.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665373
Fozo
The fabM gene product of Strep ...
Streptococcus mutans
J. Bacteriol.
186
4152-4158
2004
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665480
Marrakchi
A new mechanism for anaerobic ...
Streptococcus pneumoniae
J. Biol. Chem.
277
44809-44816
2002
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2
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1
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666947
Leesong
Structure of a dehydratase-iso ...
Escherichia coli
Structure
4
253-264
1996
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1
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5819
Guerra
Escherichia coli beta-hydroxyd ...
Escherichia coli
Arch. Biochem. Biophys.
280
336-345
1990
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3
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5814
Cronan
Derived amino acid sequence an ...
Escherichia coli
J. Biol. Chem.
263
4641-4646
1988
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1
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1
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5808
Endo
Mode of inhibition of beta-hyd ...
Escherichia coli
J. Biol. Chem.
245
4293-4296
1970
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1
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5803
Helmkamp
beta-Hydroxydecanoyl thioester ...
Escherichia coli
J. Biol. Chem.
244
6014-6022
1969
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1
1
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1
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1
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2
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1
1
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1
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1
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5807
Helmkamp
beta-Hydroxydecanoyl thioester ...
Escherichia coli
J. Biol. Chem.
243
3229-3231
1968
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5
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1
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4
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5
4
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665451
Rando
Mechanism of action of beta-hy ...
Escherichia coli
J. Biol. Chem.
243
5627-5634
1968
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1
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1
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5800
Kass
beta-Hydroxydecanoyl thioester ...
Escherichia coli
J. Biol. Chem.
242
4418-4431
1967
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2
1
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2
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1
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1
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1
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2
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1
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1
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1
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