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Literature summary for 5.3.3.14 extracted from
- beta-Hydroxydecanoyl thioester dehydrase. Studies on molecular structure and active site (1969), J. Biol. Chem., 244, 6014-6022.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-decynoyl-N-acetylcysteamine | covalent modification of ative site residue H70 | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value gradually decreases with decreasing pH-value | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
2 * 18000, SDS-PAGE | Escherichia coli |
| 36000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
multifunctional enzyme, catalyzing in addition reaction of EC 4.2.1.60 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-3-decenoyl-N-acetylcysteamine | - |
Escherichia coli | trans-2-decenoyl-N-acetylcysteamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 18000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
cis-3-decenoyl-N-acetylcysteamine | pH 9.0, 30°C | Escherichia coli | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 10.2 | more than 50% of activity maximum within this range | Escherichia coli |
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