Cloned (Comment) | Organism |
---|---|
into the vector pGEX-6P-1 for expression in Escherichia coli cells | Cutibacterium acnes |
Protein Variants | Comment | Organism |
---|---|---|
F168G/G169F | inactive mutant | Cutibacterium acnes |
F168L | inactive mutant | Cutibacterium acnes |
F193A | mutant | Cutibacterium acnes |
R88A | inactive mutant | Cutibacterium acnes |
R88S | mutant | Cutibacterium acnes |
R88S/F193A | mutant | Cutibacterium acnes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | (11S-2H) linoleic acid, 0.00408, wild-type PAI | Cutibacterium acnes | |
additional information | - |
additional information | [11,11-2H2] linoleic acid, 0.00228, wild-type PAI | Cutibacterium acnes | |
0.00482 | - |
linoleic acid | wild-type PAI | Cutibacterium acnes | |
0.02577 | - |
linoleic acid | mutant R88S/F193A | Cutibacterium acnes | |
0.03794 | - |
linoleic acid | mutant F193A | Cutibacterium acnes | |
0.0657 | - |
linoleic acid | mutant R88S | Cutibacterium acnes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutibacterium acnes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cutibacterium acnes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleic acid | - |
Cutibacterium acnes | ? | - |
? | |
linoleic acid methyl ester | - |
Cutibacterium acnes | (10E,12Z)-octadeca-10,12-dienoic acid methyl ester | - |
? | |
linoleoyl-CoA | - |
Cutibacterium acnes | (10Z,12Z)-octadeca-10,12-dienoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAI | - |
Cutibacterium acnes |
polyunsaturated fatty acid double bond isomerase | - |
Cutibacterium acnes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
kinetic assay | Cutibacterium acnes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | (11S-2H) linoleic acid, 3.51, wild-type PAI | Cutibacterium acnes | |
additional information | - |
additional information | [11,11-2H2] linoleic acid, 0.83, wild-type PAI | Cutibacterium acnes | |
0.16 | - |
linoleic acid | mutant R88S/F193A | Cutibacterium acnes | |
0.33 | - |
linoleic acid | mutant F193A | Cutibacterium acnes | |
2.93 | - |
linoleic acid | mutant R88S | Cutibacterium acnes | |
4.37 | - |
linoleic acid | wild-type PAI | Cutibacterium acnes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity and kinetic assay | Cutibacterium acnes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Cutibacterium acnes |