Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme Y30F/Y55F/Y115F/D38N KSI complexed with equilenin, hanging drop vapor diffusion method, using 0.1 M sodium acetate, pH 4.5, 0.6 M ammonium acetate, and 30% PEG 4000 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
Y115F | the mutant shows about 68% activity compared to the wild type enzyme | Pseudomonas putida |
Y14F | the mutant shows almost no activity compared to the wild type enzyme | Pseudomonas putida |
Y30F | the mutant shows about 84% activity compared to the wild type enzyme | Pseudomonas putida |
Y30F/Y55F | the mutant shows about 50% activity compared to the wild type enzyme | Pseudomonas putida |
Y30F/Y55F/Y115F | the mutant shows about 57% activity compared to the wild type enzyme | Pseudomonas putida |
Y55F | the mutant shows about 17% activity compared to the wild type enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
androstenolone | - |
Pseudomonas putida | |
equilenin | - |
Pseudomonas putida | |
estrone | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0171 | - |
5-androstene-3,17-dione | mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
0.023 | - |
5-androstene-3,17-dione | mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
0.0417 | - |
5-androstene-3,17-dione | mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
0.0499 | - |
5-androstene-3,17-dione | wild type enzyme, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
0.0502 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
0.0552 | - |
5-androstene-3,17-dione | mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
0.0773 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P07445 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-Androstene-3,17-dione | - |
Pseudomonas putida | 4-Androstene-3,17-dione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA5-3-ketosteroid isomerase | - |
Pseudomonas putida |
KSI | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.3 | - |
5-androstene-3,17-dione | mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
3510 | - |
5-androstene-3,17-dione | mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
10700 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
12200 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
14500 | - |
5-androstene-3,17-dione | mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
17800 | - |
5-androstene-3,17-dione | mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
21000 | - |
5-androstene-3,17-dione | wild type enzyme, at pH 7.0, temperature not specified in the publication | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
780 | - |
5-androstene-3,17-dione | mutant enzyme Y14F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
150000 | - |
5-androstene-3,17-dione | mutant enzyme Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
160000 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F/Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
210000 | - |
5-androstene-3,17-dione | mutant enzyme Y30F/Y55F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
330000 | - |
5-androstene-3,17-dione | mutant enzyme Y30F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
350000 | - |
5-androstene-3,17-dione | mutant enzyme Y115F, at pH 7.0, temperature not specified in the publication | Pseudomonas putida | |
430000 | - |
5-androstene-3,17-dione | wild type enzyme, at pH 7.0, temperature not specified in the publication | Pseudomonas putida |