Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
Crystallization (Comment) | Organism |
---|---|
crystals are soaked with inhibitor 2-oxo-4-phenyl-3-butynoate | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
E16A | kinetics similar to wild-type | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxo-4-phenyl-3-butynoate | irreversible inactivation by covalent modifiaction of N-terminal proline residue via a Michael-addition of proline to C4 of the inhibitor | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-(hydroxyphenyl)pyruvate | mutant E16A, pH 6.8 | Mus musculus | |
0.16 | - |
enol-phenylpyruvate | mutant E16A, pH 6.8 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P34884 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(hydroxyphenyl)pyruvate | enol form | Mus musculus | 4-(hydroxyphenyl)pyruvate | keto form | ? | |
enol-phenylpyruvate | - |
Mus musculus | keto-phenylpyruvate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
4-(hydroxyphenyl)pyruvate | mutant E16A, pH 6.8 | Mus musculus | |
200 | - |
enol-phenylpyruvate | mutant E16A, pH 6.8 | Mus musculus |