Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudothermotoga lettingae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64 | - |
D-psicose | at pH 8.0 and 75°C | Pseudothermotoga lettingae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 1 mM Co2+, Mn2+, Mg2+, Ni2+, Zn2+, Ca2+, Cu2+, and EDTA do not influence enzyme activity | Pseudothermotoga lettingae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16015 | - |
2 * 16015, calculated from amino acid sequence | Pseudothermotoga lettingae |
16800 | - |
2 * 16800, SDS-PAGE | Pseudothermotoga lettingae |
35200 | - |
gel filtration | Pseudothermotoga lettingae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | Pseudothermotoga lettingae | - |
D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | Pseudothermotoga lettingae TMO | - |
D-ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudothermotoga lettingae | - |
- |
- |
Pseudothermotoga lettingae TMO | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography | Pseudothermotoga lettingae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
after 9.1fold purification, at pH 8.0 and 75°C | Pseudothermotoga lettingae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | the enzyme convertes D-psicose to D-allose maximally at 75°C and pH 8.0 with a 32% conversion yield | Pseudothermotoga lettingae | D-allose | - |
? | |
D-psicose | the enzyme convertes D-psicose to D-allose maximally at 75°C and pH 8.0 with a 32% conversion yield | Pseudothermotoga lettingae TMO | D-allose | - |
? | |
D-ribose 5-phosphate | - |
Pseudothermotoga lettingae | D-ribulose 5-phosphate | - |
? | |
D-ribose 5-phosphate | - |
Pseudothermotoga lettingae TMO | D-ribulose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 16800, SDS-PAGE | Pseudothermotoga lettingae |
homodimer | 2 * 16015, calculated from amino acid sequence | Pseudothermotoga lettingae |
Synonyms | Comment | Organism |
---|---|---|
RPI | - |
Pseudothermotoga lettingae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Pseudothermotoga lettingae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
the half-life at 75°C is 3.3 h | Pseudothermotoga lettingae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
D-psicose | at pH 8.0 and 75°C | Pseudothermotoga lettingae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudothermotoga lettingae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 80% of its maximal activity is maintained at pH values from 6.0 to 9.0 | Pseudothermotoga lettingae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
D-psicose | at pH 8.0 and 75°C | Pseudothermotoga lettingae |