Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | elevates the catalytic efficiency of the enzyme. The binding site of AMP in the enzyme is reported | Pyrococcus horikoshii OT3 | |
AMP | presence of AMP provides structural stability to the protein | Pyrococcus horikoshii | |
GMP | elevates the catalytic efficiency of the enzyme to a lower degree than AMP. The binding site of AMP in the enzyme is reported | Pyrococcus horikoshii OT3 | |
GMP | GMP binds to the AMP binding site and an additional binding site | Pyrococcus horikoshii |
Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
expression in Rosetta (DE3) Escherichia coli cells | Pyrococcus horikoshii OT3 |
Crystallization (Comment) | Organism |
---|---|
crystals are obtained in hanging-drop vapor-diffusion method at 20°C within a period of 2-7 days | Pyrococcus horikoshii OT3 |
wild-type and mutants C135S and D204N, in complex with AMP and with GMP | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
C135S | mutation of catalytic residue, inhibits binding of substrate ribose 1,5-bisphosphate. Mutation does not not alter the binding behavior of AMP to the protein | Pyrococcus horikoshii |
D204N | mutation of catalytic residue. Mutation does not not alter the binding behavior of AMP to the protein | Pyrococcus horikoshii |
General Stability | Organism |
---|---|
the AMP binding site in PH0208 protein clarifies the role of AMP in providing structural stability to the enzyme. The binding of GMP to the AMP binding site in addition to its own binding site indicates that GMP might also execute a similar function, though with less specificity | Pyrococcus horikoshii OT3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O57947 | - |
- |
Pyrococcus horikoshii DSM 12428 | O57947 | - |
- |
Pyrococcus horikoshii OT3 | O57947 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii OT3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-ribose 1,5-bisphosphate | - |
Pyrococcus horikoshii OT3 | D-ribulose 1,5-bisphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PH0208 | - |
Pyrococcus horikoshii |
PH0208 | - |
Pyrococcus horikoshii OT3 |
R15Pi | - |
Pyrococcus horikoshii OT3 |