Application | Comment | Organism |
---|---|---|
industry | as D-allose and D-psicose are rare sugars, existing in small amounts in nature, Pseudomonas stutzeri L-rhamnose isomerase is exploited for industrial applications in rare sugar production | Pseudomonas stutzeri |
Cloned (Comment) | Organism |
---|---|
a plasmid, pOI-02, encoding the L-RhI gene, is used | Pseudomonas stutzeri |
Crystallization (Comment) | Organism |
---|---|
the structures of Mn2+-bound L-rhamnose isomerase, of the mutant S329K in complex with D-psicose or L-rhamnose, and of the mutant D327N in complex with D-psicose or L-rhamnulose are determined at resolutions of 1.8, 1.6, 1.95, 1.9, and 1.7 A, respectively | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
D327N | inactive mutant | Pseudomonas stutzeri |
S329K | inactive mutant | Pseudomonas stutzeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Pseudomonas stutzeri | |
Cu2+ | - |
Pseudomonas stutzeri | |
Mn2+ | - |
Pseudomonas stutzeri | |
Zn2+ | - |
Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Rhamnose | Pseudomonas stutzeri | - |
L-Rhamnulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | Q75WH8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Pseudomonas stutzeri | D-psicose | - |
? | |
L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Pseudomonas stutzeri |
Synonyms | Comment | Organism |
---|---|---|
L-rhamnose isomerase | - |
Pseudomonas stutzeri |